The presented work focuses on synthesis and comparison of spectroscopic properties of new fluorescent dyes - benzanthrone derivatives containing substituted amino, azo, imino or amidino groups at the 3-position, which show different luminescence characteristics. The spectroscopic behaviour of the obtained substances depends on the polarity of medium, namely their spectral position of absorption and emission, fluorescence intensity demonstrate distinct behaviour in different solvents. Many compounds show a strong fluorescent solvatochromism and a large Stoke’s shift.