Isolation of proteins from available biological resources is the matter of interest both for scientific and practical approach. To substitute conventional isolation methods by more selective sorption technique, appropriate sorbent has been used which was developed on the base of acrylic monomers and contained methacrylic and acrylic acid units. Sorption of lysozyme from model binary solution took place with high selectivity under experimental conditions, the results of which were used for lysozyme isolation from diluted hen egg white. Protein(s) sorption onto carboxylic ion-exchanger at optimal condition in static regime and desorption in dynamic downstream regime allowed isolating lysozyme containing fraction with lytic activity against Micrococcus lysodeikticus achieving 68.8 – 75% of the standard lysozyme activity.