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Publikācija: Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation

Publication Type Scientific article indexed in SCOPUS or WOS database
Funding for basic activity Unknown
Defending: ,
Publication language English (en)
Title in original language Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation
Field of research 1. Natural sciences
Sub-field of research 1.4 Chemical sciences
Authors N. Kronqvist
M. Otikovs
V. Chmyrov
G. Chen
M. Andersson
K. Nordling
M. Landreh
M. Sarr
H. Jörnvall
S. Wennmalm
J. Widengren
Q. Meng
A. Rising
D. Otzen
S.D. Knight
Kristaps Jaudzems
J. Johansson
Keywords
Abstract The mechanisms controlling the conversion of spider silk proteins into insoluble fibres, which happens in a fraction of a second and in a defined region of the silk glands, are still unresolved. The N-terminal domain changes conformation and forms a homodimer when pH is lowered from 7 to 6; however, the molecular details still remain to be determined. Here we investigate site-directed mutants of the N-terminal domain from Euprosthenops australis major ampullate spidroin 1 and find that the charged residues D40, R60 and K65 mediate intersubunit electrostatic interactions. Protonation of E79 and E119 is required for structural conversions of the subunits into a dimer conformation, and subsequent protonation of E84 around pH 5.7 leads to the formation of a fully stable dimer. These residues are highly conserved, indicating that the now proposed three-step mechanism prevents premature aggregation of spidroins and enables fast formation of spider silk fibres in general.
DOI: 10.1038/ncomms4254
Hyperlink: http://www.nature.com/ncomms/2014/140210/ncomms4254/full/ncomms4254.html 
Reference Kronqvist, N., Otikovs, M., Chmyrov, V., Chen, G., Andersson, M., Nordling, K., Landreh, M., Sarr, M., Jörnvall, H., Wennmalm, S., Widengren, J., Meng, Q., Rising, A., Otzen, D., Knight, S., Jaudzems, K., Johansson, J. Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation. Nature Communications, 2014, No.5, pp.3254-3254. e-ISSN 2041-1723. Available from: doi:10.1038/ncomms4254
Additional information Citation count:
ID 19204