The objective of the paper was to enhance the theoretical analysis of vibration data by using contemporary programming tools and to investigate different methods of using built-in MATLAB functions in the National Instruments (NI) LabVIEW system design software. The data for analysis were obtained from vibrating systems under dynamic loading, and the MATLAB code was developed specifically for hydropower turbine vibration monitoring activities. The dynamic cyclic load conditions analyzed in the given paper were observed on a hydropower turbine resulting from the generator drive, hydraulic pressure and shock. Various data sets available for the analysis were experimentally obtained from the dynamically loaded object using the vibration monitoring system and specific rotor air gap measuring system. The given paper consists of description of the observed mechanical model and vibration equations included in the analysis of dynamic behavior of the hydropower turbine and subsections, aimed to integrate the MATLAB code into the existing monitoring system and compare the analysis accomplished thereof with the harmonics analysis obtained from LabVIEW without using the particular code.