Patient-specific auricular prostheses from biocompatible non-biodegradable polyurethane with biomimetic mechanical properties have been developed and fabricated using 3D printing technology. Mechanial investigation of printed samples using 3- point bending test demonstrated that printed prostheses are mechanically similar to natural human auricular cartilage. After subcutaneous implantation to rats auricular prestheses were able to maintain their shape and initial sizes. Thus, 3D printing allows to fabricate patent-specific biocompatible auricular prostheses with biomimetic mechanical properties and capabilities to maintain initial shape and sizes after implantation in vivo.