Most of the solar cell parameters (short-circuit current, fill factor, power conversion efficiency) can only be determined by creating and measuring the solar cell. However, there is an empirical relation that links energy level values of the materials in the active layer to an open-circuit voltage (Uoc) of the solar cell. Due to a variety of possible methods used to determine energy level values and the dispersion of obtained results, this estimate is not always correct. Even if correct energy level values are obtained for separate materials, energy level shift takes place at the interfaces when two materials are mixed. That is why a simple and reliable experi-mental method for Uoc estimation is required. Usually, photoconductivity is used to obtain the energy gap between molecule ionization energy and electron affinity of a single material. When two materials are mixed, direct charge transfer from donor to acceptor molecule can be observed. The threshold energy (ECT) shows the real difference between donor molecule ionization energy and acceptor molecule electron affinity. This difference should correspond to the Uoc. The present study makes the comparison between the open-circuit voltage estimated from material energy level values, the obtained ECT values for various donor:acceptor systems, and the real Uoc obtained from solar cell measurements. Strong correlation between ECT and Uoc is obtained and the photoconductivity measurements can be used in the estimation of Uoc.