The solution structures of self-complementary DNA hexamers with ApT or TpA base steps on each end and with CpG or GpC base steps in the center resembling the biologically important “E-box” sequences were determined. All four hexamers form the antiparallel-stranded right-handed duplexes. The 1H-NMR spectra acquired at higher temperatures (up to 25° C) showed that all duplexes are stable. The base-pair step parameters were calculated for four middle steps of all duplexes. The analysis of macroscopic curvature gave order TACGTA>ATGCAT>ATCGAT>TAGCTA. It was proposed that the increased macroscopic curvature of sequence already in uncomplexed state allows for easier energy – saved interactions with proteins or drugs.