Studies of UV-A Inactivation of Escherichia Coli by 365 nm Light

19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics: Proceedings of NBC 2023. IFMBE Proceedings. Vol.89 2023
Gita Rēvalde, Anna Zajakina, Karina Spunde, Žanna Rudēvica, Atis Skudra

In this work, we tested the effect of intense UV-A irradiation at 365 nm on Escherichia coli at different irradiation times. In parallel, after the exposure to UV-A light, the creation of reactive oxygen species in bacteria using cellular reactive oxygen species detection assay with 2′,7′-dichlorodihydrofluorescein diac-etate was measured. The first results showed very effective inactivation (5 log10) of Escherichia coli after 15 min of exposure resulting in a dose of 412 J/cm2, and subsequent increased production of cellular reactive oxygen species within 1 to 4 h after irradiation.

Keywords
UV-A inactivation, disinfection, Escherichia Coli, ROS, 365 nm, oxygen species
DOI
10.1007/978-3-031-37132-5_7
Hyperlink
https://link.springer.com/chapter/10.1007/978-3-031-37132-5_7

Rēvalde, G., Zajakina, A., Spunde, K., Rudēvica, Ž., Skudra, A. Studies of UV-A Inactivation of Escherichia Coli by 365 nm Light. In: 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics: Proceedings of NBC 2023. IFMBE Proceedings. Vol.89, Latvia, Liepaja, 12-14 June, 2023. Cham: Springer, 2023, pp.50-54. ISBN 978-3-031-37131-8. e-ISBN 978-3-031-37132-5. ISSN 1680-0737. e-ISSN 1433-9277. Available from: doi:10.1007/978-3-031-37132-5_7

Publication language
English (en)