The biomechanical activity of the vocal cords is reflected in the fundamental frequency of the voice (F0), which changes in relation to the speaker's emotional state. The aims of the study were to determine: Whether F0 changes in relation to the structural complexity of the linguistic unit being spoken. Whether F0 differs in words, phrases, and connected speech spoken with neutral, happy, and angry intonations. Ten professional actors recorded multiple voice samples in neutral, happy, and angry intonations. A panel of six experts analyzed 810 voice samples, selecting those where affective emotions were most pronounced. The selected 270 voice samples were analyzed using the PRAAT software (v.6.1.31). Statistically significant F0 changes were observed in men as the structural complexity of linguistic units spoken in a neutral intonation increased. The highest F0 was observed in women using a happy intonation and in men using an angry intonation. Statistically significant differences in F0 between words and phrases spoken in neutral and affective prosody were observed in respondents of both genders. It can be concluded that emotional prosody does not affect F0 changes in words, phrases, and connected speech. The relationship between emotions and voice is confirmed by an increase in F0 during the use of affective prosody.