Novel polysaccharides have emerged as promising candidates for various biomedical and food applications due to their unique properties. In this study, selected marine-derived, polysaccharide extracts (i.e., furcellarans) from the red macroalga Furcellaria lumbricalis (FL) were characterized using HP-SEC, HPAEC-PAD, FTIR and 1H NMR spectroscopy. Polysaccharide fractions isolated from FL constituted approximately 23.7 % of the dry biomass, with yield markedly influenced by extraction parameters, particularly temperature and duration. Compositional analyses revealed that the 1B cold-extracted fractions exhibited comparatively higher levels of non-polysaccharidic constituents, including residual proteins, phenolic compounds, indicative of reduced polysaccharide purity under these conditions. Functional assessments further characterized these fractions in terms of their immunomodulatory potential, specifically evaluating their capacity to promote wound healing and exert anti-inflammatory effects. Furcellaran cold and hot-extracted fractions FL-2B exhibited pro-inflammatory activities, and actively promoted phagocytosis. The cold fraction significantly accelerated phagocytosis (1 A: 132.7 ± 6.8 %, p < 0.01; 1B: 126.7 ± 7.5 %, p < 0.01). Notably, fraction FL-3A (0.5 μg/μL) enhanced cell proliferation and migration in HDF and HaCaT cells. Both cold and hot fractions were non-cytotoxic to Caco-2 cells; however, FL-1B and FL-2B induced the expression of inflammatory genes (TNF-α, IL-8) in Caco-2 cells, leading to subsequent inflammatory effects. All the furcellaran fractions showed weak anti-coagulant activity, as determined by aPTT assays. The findings of this study indicate that the cold-extracted fraction FL-1A, along with the hot-extracted fractions FL-2B, FL-3A, and FL-3B, exhibited a safety profile compatible with applications in functional foods or dietary supplements and possess properties conducive to promoting gastrointestinal health. In particular, the FL-3A extract demonstrated a pronounced capacity to stimulate the proliferation and migratory activity of dermal and immune cell populations, underscoring its potential utility in the development of immunomodulatory therapeutic interventions.