The ability to quantify surface mechanical properties is valuable for assessing the quality of thermal spray coatings. This is especially important for prostheses where loading is placed directly on the surface. Hydroxyapatite was classified to small (20–40 μm), medium (40–60 μm) and large (60–80 μm) particle sizes and thermal sprayed to produce a coating from spread solidified hydroxyapatite droplets. It was revealed for the first time, that nanoindentation can be successfully used to determine the hardness and elastic modulus on the surface of well spread solidified droplets at the hydroxyapatite coating surface. Comparison with indentation results from polished cross-section exhibited comparable values and statistical variations. The hardness was 5.8±0.6, 5.4±0.5 and 5.0±0.6 GPa on coatings produced from small, medium and large sized powder. Similarly, the elastic modulus decreased from 121±7, 118±7 to 114±7 GPa, respectively. Use of several indentation loads gave comparable results with sintered hydroxyapatite suggesting good inter-splat bonding within the coating. MicroRaman spectroscopy and X-ray diffraction confirmed a larger degree of dehydroxylation for the smaller particles also revealing a lower elastic modulus. This shows the influence of particle size and possibly dehydroxylation of hydroxyapatite on the mechanical properties of the coating surface.