Bioceramic Hydroxyapatite Granules for Purification of Biotechnological Products
Journal of Advanced Materials Research: Materials and Design
2011
Vitālijs Lakevičs,
Jānis Ločs,
Dagnija Loča,
Valentīna Stepanova,
Līga Bērziņa-Cimdiņa,
Juris Pelšs
Sorption experiments of bovine serum albumin (BSA) on hydroxyapatite (HAp) ceramic granules, prepared at three temperatures 900°C, 1000°C and 1150°C were performed at room temperature 18,6 °C and phosphate buffer, pH 5,83; 6.38 and 7,39. Thermal treatment contributed to the decrease of bovine serum albumin immobilization indicating that sorption process depended on HAp ceramics specific surface area and pH values of phosphate buffer solution. However, it was confirmed that granule size was also an important parameter for bovine serum albumin adsorption. As a result of these experiments, the most appropriate adsorption conditions and phosphate buffer pH values influence on to BSA sorption were analyzed.
Atslēgas vārdi
Adsorption, Bovine Serum Albumin, Granules, Hydroxyapatite Ceramics
DOI
10.4028/www.scientific.net/AMR.284-286.1764
Lakevičs, V., Ločs, J., Loča, D., Stepanova, V., Bērziņa-Cimdiņa, L., Pelšs, J. Bioceramic Hydroxyapatite Granules for Purification of Biotechnological Products. Journal of Advanced Materials Research: Materials and Design, 2011, Vol.284-286 , 1764.-1769.lpp. ISSN 1662-8985. Pieejams: doi:10.4028/www.scientific.net/AMR.284-286.1764
Publikācijas valoda
English (en)