Isolation of Lysozyme from Hen Egg White Using Ter-Polymeric Carboxylic Ion-Exchanger
Abstracts of the 52nd International Scientific Conference of Riga Technical University : Section: Material Science and Applied Chemistry 2011
Valentīna Krilova, Inga Jurgelāne, Visvaldis Vītiņš

To substitute conventional isolation methods by more selective sorption technique, the developed sorbent on the base of acrylic monomers has been used. Synthesized ter-polymeric cation-exchanger K-120 contains large amount of functional groups – 10.2 mekv/g and moderately swell in water (specific swelling - 3.2 ml/g). The regularities of lysozyme sorption onto synthesized carboxylic ion-exchanger reflux electrostatic interaction between two types of functional groups of the last and basic protein – lysozyme. Lysozyme isolation from HEW was performed using combined technique: i) sorption stage was done in static condition in closed agitated contactor and ii) for desorption sorbent was transferred into column where protein(s) were eluted by downstream process. Taking into account the practical aspect, experiments for lysozyme isolation from HEW have been conducted using different ratios of K-120 and HEW medium. The desorbed proteins were mostly concentrated already in the first eluted volume. The content of lysozyme in eluted fractions was evaluated by their lytic activity against Micrococcus lysodeiktikus cells. Sorption capacity of the sorbent towards lysozyme from HEW achieved 112 mg/g.


Atslēgas vārdi
Carboxylic ion-exchanger, lyzozyme, sorption

Krilova, V., Dušenkova, I., Vītiņš, V. Isolation of Lysozyme from Hen Egg White Using Ter-Polymeric Carboxylic Ion-Exchanger. No: Abstracts of the 52nd International Scientific Conference of Riga Technical University : Section: Material Science and Applied Chemistry, Latvija, Rīga, 13.-15. oktobris, 2011. Riga: RTU Publishing House, 2011, 98.-98.lpp. ISBN 9789934101816.

Publikācijas valoda
English (en)
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