The research objects are wound care materials from poly (vinyl alcohol) (PVA) gels obtained by freezing-thawing method. The content of therapeutic substances in composite gels which does not disturb cross-linking in freezing-thawing cycles was determined primarily. The promotion of quick wound healing without damage of new epithelium cells is one of a most important feature of wound care; therefore the natural substances such as a paste of pine extract has been selected for the further researches. Lot of microorganisms which frequently are representatives of natural flora, int. al. S.epidermidis, Ps.aeruginosa, present high ability to colonize on biomaterials, thereby developing risk of hospital infection. In this work an attempt was made for assessment of various PVA matrices, their microbial contamination risk and adhesion activity, study of their cytotoxicity and biocompatibility for the use of gel materials for the wound care.