Novel Concepts of “Niche-Relief” and Niche-Voltage” for Stem Cells as a Base of Bone and Hematopoietic Tissues Biomimetic Engineering

IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics (ISBEMP 2012) 2013
Jurijs Dehtjars, I. Khlusov, M. Khlusova, E. Gostichev, Yu. Sharkeev, V. Pichugin

An affect of relief features and quantitative variables of artificial surfaces on structural-functional status of human lung prenatal stromal cells (HLPSC) and remodeling of Balb/c mice bone/bone marrow system have been studied. Implants with rough (Ra>2 μm) calcium phosphate micro-arc coatings have structural-functional sites (micro-regions) named “niches-relief” which are necessary for maturation and differentiation of HLPSC into secreting osteoblasts in shortterm culture. Maximal remodeling of mouse bone/bone marrow system in 45-day subcutaneous heterotopic test in vivo is also noted under optimal parameter (average index of cellular alkaline phosphatase area to artificial micro-region area is about 43 %) of osteogenic niche in vitro. Probable physical mechanism of osteogenic niche functioning has been determined. It correlates with increasing of electron work function supplied with a negative charge of calcium phosphate nanorelief in the sockets (artificial “niches”). “Niche-voltage” concept for bone tissue biomimetic engineering was proposed.

Atslēgas vārdi
niche-relief, niche-voltage, hematopoietic
DOI
10.1007/978-3-642-34197-7_26
Hipersaite
http://rd.springer.com/chapter/10.1007/978-3-642-34197-7_26

Dehtjars, J., Khlusov, I., Khlusova, M., Gostichev, E., Sharkeev, Y., Pichugin, V. Novel Concepts of “Niche-Relief” and Niche-Voltage” for Stem Cells as a Base of Bone and Hematopoietic Tissues Biomimetic Engineering. No: IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics (ISBEMP 2012), Latvija, Riga, 10.-12. oktobris, 2012. Berlin: Springer Berlin Heidelberg, 2013, 99.-102.lpp. ISBN 978-3-642-34196-0. e-ISBN 978-3-642-34197-7. ISSN 1680-0737. e-ISSN 1433-9277. Pieejams: doi:10.1007/978-3-642-34197-7_26

Publikācijas valoda
English (en)