this paper evaluates the cleaning efficiency of the glycolipid based anionic biosurfactant produced by the local company. Before use in the practical application of oil removal or other hydrophobic substances from soil, the knowledge on the behavior of the biosurfactants across different systems is necessary. As the process variables the temperature of environment, contact time with the dilution of biosurfactant and the concentration of the biosurfactant in a washing solution is be modeled by the application of full factorial design. As the response cleaning efficiency was obtained experimentally by a set of the laboratory tests. The screening design is employed for the evaluation of the interactions between the response variable and the process variables. The cleaning efficiency showed various results depending on the initial values of the variables. At the upper limit of the variables (+ 35 ºC for the temperature of environment, 15 minutes contact time with the dilution of the biosurfactant and 0.3 wt% concentration of the biosurfactant in a washing solution) the cleaning efficiency was 99.32 %. The results showed that all variable had significant effects on the cleaning efficiency at the 95 % confidence level.