A simple, fast and reproducible analytical method for fractionation of freely available bryophyte lipids was developed. Lipid fractionation was achieved by solid phase extraction using porous silica and silica derivatized with -NH2 groups and the compounds were quantified as their trimethylsilyl derivatives by gas chromatography-mass spectrometry. The suggested fractionation method allows to identify several groups of substances prospective in respect of search of new biologically active compounds in bryophytes as well as in respect of understanding of their metabolism.