Silk fibroin can be derived from the silkworm Bombyx mori, and it has the properties for use as bioadhesive biomaterial in medicine – biocompatibility, good mechanical properties, and controllable degradation rate. Hyaluronic acid (HA) is an attractive polymer for biomedical applications due to its biological and structural importance as well as ease of modification. In this study, two types of raw silk were used for preparation of silk fibroin (SF) solutions. Obtained SF solutions (with and without HA) were cross-linked to form hydrogels. Glutaraldehyde (GTA) was used in this study as cross-linking agent. Two temperatures (37 °C and 60 °C) were chosen to determine the effect of temperature on the cross-linking rate of the samples. Gelation time, swelling ratio, and structural features of the adhesive were also studied.