Cladding-pumped erbium (Er3+)/ytterbium (Yb3+)-co-doped fiber amplifiers are more advantageous at high output powers. However, this amplification technique also has potential in telecom-related applications. These types of amplifiers have complex properties, especially when considering gain profile and a pump conversion efficiency. Such metrics depend on the doped fiber profile, absorption/emission spectra, and the input signal power. In this context, we design, build and characterize an inhouse prototype of cladding-pumped Er3+/Yb3+-co-doped fiber amplifier (EYDFA). Our goal is to identify the EYDFA configuration (a co-doped fiber length, pump power, input signal power) suitable for signal amplification in a multichannel fiber-optic transmission system with a dense wavelength allocation across the C-band (1530–1565 nm). Our approach involves experimentally determining the Er3+/Yb3+-co-doped fiber’s parameters to be used in a simulation setup to decide on an initial EYDFA configuration before moving to a laboratory setup. An experimental EYDFA prototype is tested under different conditions using a 48-channel dense wavelength division multiplexing (DWDM, 100 GHz) system to evaluate the absolute gain and gain uniformity. The obtained results allow the cladding pump amplifier’s suitability for wideband signal amplification to be assessed. The developed prototype provides > 21 dB of gain with a 12 dB ripple within 1534–1565 nm. Furthermore, we show that the gain profile can be partially flattened out by using longer EYDF spans. This enhances signal amplification in the upper C-band in exchange for a weaker amplification in the lower C-band, which can be marginally improved with higher pump powers.