Shift towards greener transport technologies is facilitated by various stakeholders and groups via the legislation (as the EU Green Deal) and individual preferences. Last-mile freight delivery technologies experience significant changes in applied technologies and potentially applicable technologies as drones, automatic transport robots, automatic trucks. However, a limited research attention is paid on the impact on all other economic activities (multi-sectoral approach) and regional impact as many studies purely focus on solutions, limited sectors, city, or region where applied. Hence the aim is to estimate the potential impact on economic activities and regional development caused by the shift towards greener and more sustainable transport technologies in last-mile delivery in Latvia. The applied elaborated modified input-output model with regional block models the sectoral impact according to NACE 64 economic activities, using bottom-up approach computed results for major 10 sectors and two regional perspectives: NUTS 3 (6 regions) and urban-rural typology (3 territories). The results claim that overtaking and applying in postal and courier services (H53) representing last-mile delivery amid NACE classification leads to a notable impact on various service economic activities rather manufacturing, and due to geographical location of production, then NUTS 3 regions and urban-rural typology territories are modelled. The results indicate high sensitivity to modelling assumptions and selected benchmark country, nonetheless valuable as additional information for urban planning, regional transport, and economic stimulus policy elaboration for broader scale of impacts caused.