eaweeds are gaining significant attention for their bioactive compounds, which hold great potential for use in food, cosmetics, and pharmaceuticals. To avoid the use of toxic substances in the extraction process, there is a need for innovative and eco-friendly methods to exploit the highly potent raw seaweed biomass. Described herein are the datasets of how the particle size reduction of seaweeds positively enhanced the efficacy of green extraction in boosting the extraction yields of seaweed bioactive compounds. Different green extraction approaches were used to accumulate different seaweed particle sizes that were collected via grinding and sieving. The total yields of carbohydrates, glucuronic acids, proteins, phenolics and flavonoids were quantified to evaluate the efficacy of the extraction strategies. The efficacy and safety usages of the extracts were assessed using different pathogenic bacterial strains and human cell lines, respectively.